Coding

Part:BBa_K669005:Design

Designed by: Silvia J Cañas   Group: iGEM11_BACATA-COLOMBIA   (2011-09-28)


CBP + promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 276
    Illegal SpeI site found at 573
    Illegal PstI site found at 119
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 276
    Illegal SpeI site found at 573
    Illegal PstI site found at 119
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 276
    Illegal SpeI site found at 573
    Illegal PstI site found at 119
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 276
    Illegal SpeI site found at 573
    Illegal PstI site found at 119
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplified by PCR from V. fischeri ES114. Due to the presence an internal of PstI restriction site, this part was first inserted in pGEM T Easy to facilitate its construction.


Source

Vibrio fischeri ES114

References